Dernière mise à jour le : 20 août 2015

Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing


Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together, while communities from the same site but different seasons were unrelated. Both DNA and RNA-based surveys provided similar relative abundances for most class-level taxonomic groups. Yet, particular groups were overrepresented in one of the two templates, such as marine alveolates (MALV)-I and MALV-II that were much more abundant in DNA surveys. Overall, the groups displaying the highest relative contribution were Dinophyceae, Diatomea, Ciliophora and Acantharia. Also, well represented were Mamiellophyceae, Cryptomonadales, marine alveolates and marine stramenopiles in the picoplankton, and Monadofilosa and basal Fungi in sediments. Our extensive and systematic sequencing of geographically separated sites provides the most comprehensive molecular description of coastal marine protist diversity to date.

© extrait du document :
"[...] Marine samples were collected through the BioMarKs project ( in coastal sites near [...] Roscoff (Western English Channel, France)[...]. Some sites are longterm observatories, the Blanes Bay Microbial Observatory (BBMO), the Long Term Ecological Research station MareChiara (Naples), and the SOMLIT - Astan site (Roscoff).[...]"


keyboard_arrow_right Titre : Marine protist diversity in European coastal waters and sediments as revealed by high-throughput sequencing
keyboard_arrow_right Type de documentation : Articles
keyboard_arrow_right Auteur(s) personne(s) : Adriana Zingone , Vaulot, Daniel , Thorsten Stoeck , Simon, Nathalie , Raffaele Siano, Kamran Shalchian-Tabrizi , Sébastien Santini , Thomas Richards , Sarah Romac , Ian Probert , Massimo C Pernice , Jan Pawlowski , Hiroyuki Ogata , Fabrice Not , Frédéric Mahé , Ramiro Logares , Wiebe H.C.F. Kooistra , Olivier Jaillon , GUILLOU, Laure , Dominik Forster , Irene Forn , Bente Edvardsen , Micah Dunthorn , Dolan, J. R. , Johan Decelle , Jean-Michel Claverie , Richard Christen , CHAMBOUVET, Aurélie , Christophe Boutte , Lucie Bittner , David Bass , Stéphane Audic , Angélique Gobet , Ramon Massana
keyboard_arrow_right Date de publication : 20 août 2015
keyboard_arrow_right Nombre de pages : pp4035-4049
keyboard_arrow_right Editeur(s) : Wiley-blackwell
keyboard_arrow_right Tags thématiques : écosystème aquatique, écosystème benthique, benthos, ADN, écosystème marin, plancton, taxonomie
keyboard_arrow_right Mots-clés libres : 18S rDNA/dissolved DNA/environmental DNA and RNA extracts/marine/protist diversity/pyrosequencing/taxonomy
keyboard_arrow_right Localisation : ROSCOFF
keyboard_arrow_right Langue : Anglais
keyboard_arrow_right N° d'édition (ISBN,DOI...) : 10.1111/1462-2920.12955
keyboard_arrow_right Collection : Environmental Microbiology
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